Fig. 4: STED imaging of DBOV-Mes.
From: Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene
Confocal (a, b) and STED (c, d) images of DBOV- Mes embedded in PS film. The regions enclosed by the dashed boxes in (a, c) are magnified in the corresponding right (b, d). Scale bars: a, c, 1 µm; b, d, 200 nm. The color bars represent a linear scale. e Normalized intensity profiles along the dashed lines in (b, d). 2D-3D (2D STED beam with z-stack measurements) confocal (f) and STED (g) images of nanoscale cracks from the gridded structures in a glass substrate with DBOV-Mes. Scales, 5 µm. h STED image of the white dashed square region of (g) at an imaging depth of 4.68 μm relative to the surface of the glass substrate. Scale bars: 1 µm. i Fluorescence retention of DBOV-Mes after two sequential 2D-3D measurements obtained from ROIs in (h). All the parameters are summarized in Supplementary Note 1. After repeated 3D imaging over the same imaging volume for more than 1 h, more than 70% of its STED fluorescence intensity was retained. Source data are provided as a Source Data file.
