Fig. 2: IMM-arm subunits of CI are sequence-conserved within eukaryotic kingdoms.
a Multiple sequence alignment indicating conservation of amino acids of Ndufa2 and Ndufa1 mutated in human CI-deficiency diseases. Sequences of 13 representative organisms from metazoa, Fungi and plants aligned. Details in Supplementary Data S2f. b Stacked-bars representing conservation of 79 missense mutation involved in CI-deficiency diseases. Sequences of 13 representative organisms from metazoa, Fungi and plants indicated in (Fig. 2a) analyzed. Details in Supplementary Data S2a and S2f. c Conservation and hydrophobicity of trans-IMM helix of Ndufa1 represented. Color coded as per Goldman-Engelman-Steitz (GES) hydrophobicity scale indicating packing ability of trans-IMM helix into membrane. Black font: surface exposed amino acids; green font: buried amino acids. 1st and last amino acids indicated with number at top for each kingdom. *– identical;: – strongly similar;. – weakly-similar as per ClustalOmega convention. d Nudfa1 along with neighboring subunits from CI-structures. Homo sapiens (PDB: 5XTD), Yarrowia lipolytica (PDB: 7O71), and Arabidopsis thaliana (PDB: 7ARB) representing metazoa, Fungi and plants. Red: surface exposed atoms and amino acids; yellow: partially exposed atoms and amino acids to surface; white: buried amino acids. Initial and end amino acids of IMM helix indicated by single letter code followed by position in sequence. Light gray: neighboring IMM subunit ND1; dark gray: neighboring IMS-subunit Ndufa8. e Heatmaps representing % sequence identity of CI-subunits searched using blastp against organisms mentioned. 134 organisms from eToL considered for analysis. Sequences of Homo sapiens, Neurospora crassa and Arabidopsis thaliana from metazoa, Fungi and plants used for blast search. Gray box indicate no blastp hits. M - matrix; I – IMM; I* – IMS. Details in Supplementary Data S4a. f Schematic representing mean % identity of CI-subunits calculated from Fig. 2e. Details in Supplementary Data S4a.
