Fig. 2: Epigenome-Wide Association Study (EWAS) of current versus never smokers in Generation Scotland (n = 23 pairs). | Nature Communications

Fig. 2: Epigenome-Wide Association Study (EWAS) of current versus never smokers in Generation Scotland (n = 23 pairs).

From: A blood- and brain-based EWAS of smoking

Fig. 2

Analyses were performed using DNA methylation data obtained using the Illumina EPIC array ( ~ 850k CpG sites), the TWIST human methylation panel (4 million CpG sites, targeted short read sequencing) and Oxford Nanopore Technologies sequencing (21 million CpG sites, long read sequencing). The X-axis represents chromosomes 1–22, while the Y-axis shows −log10(P-values). The top horizontal line marks genome-wide significant associations (P < 3.6 × 10⁻⁸, red dots), based on the multiple testing threshold estimated by Saffari et al.13. The bottom horizontal line denotes the suggestive significance threshold (P < 1 × 10⁻⁵, yellow dots). Dotted vertical lines highlight loci associated with smoking status at P < 3.6 × 10⁻⁸. All statistical tests were two-sided. EPIC – Illumina EPIC array, TWIST – TWIST Biosciences Human Methylome Panel, ONT – Oxford Nanopore Technologies Sequencing.

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