Fig. 4: Localized expression of phytoalexin biosynthesis genes in response to GMI1000. | Nature Communications

Fig. 4: Localized expression of phytoalexin biosynthesis genes in response to GMI1000.

From: Comparative single-nucleus RNA-seq analysis revealed localized and cell type-specific pathways governing root-microbiome interactions

Fig. 4

ac The line charts show the number of differentially expressed genes corresponding to the maturation, elongation, and meristem zones of the root atrichoblast, cortex, and endodermis, respectively. d Overview of the phytoalexin biosynthesis pathway. eg Heatmap illustration of the Z-score normalized expression levels of genes from phytoalexin biosynthesis pathway and several immune or SA-related marker genes, including PBS3, MPK11, CBP60g, and SID2. h Validation of the maturation cell-specific induction pattern of CYP71A12 using its promoter-driven reporter line (pCYP71A12::YFP-NLS), roots were inoculated with mock (10 Mm MgSO4), GMI1000 or WCS417 (OD600 = 0.05) for 6 h. Images correspond to the meristematic zone (MZ), elongation zone (EZ), and maturation zone (MZ). The scale bar represents 50 μm. i Quantitative analysis of YFP signal intensities of the CYP71A12 reporter line after different treatments and from different regions. n = 6 from two independent experiments. Results are presented as mean ± standard error of the mean (SEM). Different letters indicate statistically significant differences (P < 0.05) between means by ANOVA and Tukey’s test. Box plots show the median (horizontal bar), 25th (bottoms of boxes), and 75th (tops of boxes) quartile range (QR), and non-outlier data value (upper and lower whiskers) of each index.

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