Fig. 3: Diverse neuronal light responses in the SCN. | Nature Communications

Fig. 3: Diverse neuronal light responses in the SCN.

From: Discrete photoentrainment of mammalian central clock is regulated by bi-stable dynamic network in the suprachiasmatic nucleus

Fig. 3

a Representative heat map with GCaMP traces (normalized Z-score) from 113 identified neurons at ZT 16 (left), ZT 22 (middle), and ZT 8 (right), respectively. b, c 2D t-SNE map (b) shows the diversity of neuronal light-responses. 2875 recording traces from 113 neurons are plotted, excluding those with excessive motion artifacts. The 2875 recordings are classified into 7 clusters, indicated by different colors, with k-means. The 7 light responses are plotted in c, including averaged traces of all clusters (upper-left panel) and raw traces (gray) with average from each cluster (the other 7 panels). Stars above or below the average traces indicate significantly higher or lower z-score compared to the -5 ~ 0 second baseline, respectively. One-way ANOVA and Tukey post hoc test, * indicates p < 0.05. d Raw traces from the representative neuron that show high diversity of light-evoked responses through 9 repeated trials from 3 time points. e Histogram of neurons shows multiple types of light responses. n = 3 animals.

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