Fig. 7: Nb14-32-Fc and its parent nanobodies provide therapeutic protection against intracranial infection of HSV-1 F strain in mice. | Nature Communications

Fig. 7: Nb14-32-Fc and its parent nanobodies provide therapeutic protection against intracranial infection of HSV-1 F strain in mice.

From: A potent protective bispecific nanobody targeting Herpes simplex virus gD reveals vulnerable epitope for neutralizing

Fig. 7

a Animal experiment scheme. In comparison of treatment efficacy of Nb14-32-Fc and its parent nanobodies, mice were divided into four groups (n = 8 per group), including a control group administered vehicle (PBS) and three treatment groups. In comparison of treatment efficacy of Nb14-32-Fc and E317, mice were divided into three groups (n = 5 per group), including a control group administered vehicle (PBS) and two treatment groups. Mice were intracranially infected with the HSV-1 F strain;12 h later, mice were administered with antibodies or PBS via an intraperitoneal route (i.p.). The mice were then sacrificed on the 4th day of infection. b, c Viral RNA copies of HSV-1 in the brain and spinal cord of mice were measured with quantitative polymerase chain reaction (qPCR) on day 4 post infection. Each data point represents an individual mouse within the respective groups. d, e Weight loss of infected mice in the control group and treatment groups. Weight loss was monitored daily. d Nb32-Fc vs PBS(p = 0.0359), Nb14-Fc vs PBS(p = 0.0016), Nb14-32-Fc vs PBS(p < 0.0001) (n = 8 biologically independent animals per group). e E317 vs PBS(p = 0.3367), Nb14-32-Fc vs PBS(p = 0.0187) (n = 5 biologically independent animals per group). Error bars represent the mean ± SEM. Data in (b–e) were analyzed by the one-way ANOVA with Dunnett’s test in the comparison to PBS treated group, revealing a significant difference with ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, p > 0.05(ns). ns, no significant. f, g Survival curves of infected mice in the control group and treatment groups. Clinical symptoms were monitored daily. Data were analyzed by the two-sided log-rank (Mantel‒Cox) test. f Nb32-Fc vs PBS(p = 0.0048), Nb14-Fc vs PBS(p = 0.0072), Nb14-32-Fc vs PBS(p = 0.0002) (n = 8 biologically independent animals per group). g E317 vs PBS(p = 0.2019), Nb14-32-Fc vs PBS(p = 0.0048). (n = 5 biologically independent animals per group). Source data are provided as a Source Data file.

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