Fig. 1: Setdb1-deficient T cells do not mediate heart allograft rejection.
From: Targeting Setdb1 in T cells induces transplant tolerance without compromising antitumor immunity
BALB/c hearts were transplanted into WT B6 and Setdb1f/fCd4-Cre mice. a Percentage of allograft survival after transplantation (n = 18). Log-rank (Mantel-Cox) test. b Representative images for hearts before transplantation (upper panel) and allografts harvested 7 days post-transplant from WT B6 and Setdb1f/fCd4-Cre mice (lower panel). c H&E-stained sections of heart allografts harvested from WT B6 recipients on day 7 post-transplant or from Setdb1f/fCd4-Cre recipients on day 7 or day 100 post-transplant. Representative images are shown. Scale bar: 200 μm. d, e Masson trichrome staining of allograft sections from WT B6 recipients treated with CTLA4-Ig on day 30 post-transplant, or from Setdb1f/fCd4-Cre recipients without treatment on day 7 or day 100 post-transplant. Representative images for each group (d) and bar graph of collagen volume fraction between WT B6 recipients (day 30) and Setdb1f/fCd4-Cre recipients (day 100). Scale bar: 200 μm. e n = 15 for each group: 5 samples per group, three representative images of each sample were quantified and conducted data analysis. Unpaired Student’s t-test (two-sided). Data are representative of three (b–d) independent experiments. Data are represented as means ± SEM of at least three (a, e) independent experiments.
