Fig. 5: Setdb1 Controls Treg Cell Development in the Thymus.
From: Targeting Setdb1 in T cells induces transplant tolerance without compromising antitumor immunity
a–f Thymocytes from WT B6 and Setdb1f/fCd4-Cre mice were processed for single-cell RNA sequencing (scRNA-seq). a Ten gene expression clusters (0–9) projected on a UMAP of the scRNA-seq libraries. b Comparation of cell density for each cluster in WT B6 (WT, red) and Setdb1f/fCd4-Cre (KO, blue) mice. c UMAP embedding displaying the thymocyte colored by pseudotime. d Projection of clusters 4 and 5 according to the position of their cells on the pseudotime. e Velocities derived from the dynamic model for Treg development, projected onto UMAP-based embedding. f Gene expression of Il1r2, Il18r1, and Irf2bp2 in cluster 4 from WT B6 and cluster 5 from Setdb1f/fCd4-Cre mice (two-sided). g Representative zebra plot of intratumoral Treg cells in WT B6 and Setdb1f/fCd4-Cre mice at day 14 post-inoculation. h Percentage of intratumoral Treg cells from WT B6 and Setdb1f/fCd4-Cre mice (n = 11 per group). i Comparison of Il18 levels for xenograft B16F1 melanoma (14 days post-inoculation) and BALB/c allografts (3 days post-transplant) (n = 8 per group). Data are representative of at least four (g) independent experiments. Data are shown as means ± SEM of at least three (h, i) independent experiments. ns, no significance; unpaired Student’s t-test (two-sided). See also Table S1, Supplementary Data 1, and Supplementary Data 2.
