Fig. 7: Setdb1 deficiency leads to a reduction in H3K9me3 and reinforces Treg-related gene expression.
From: Targeting Setdb1 in T cells induces transplant tolerance without compromising antitumor immunity
a Representative histogram for H3K4me3, H3K9me3, and H3K27me3 levels in Tregs from WT B6 and Setdb1f/fCd4-Cre mice. b Mean fluorescence intensity of H3K4me3, H3K9me3, and H3K27me3 in Tregs from WT B6 and Setdb1f/fCd4-Cre mice (n = 6 per group). Unpaired Student’s t-test (two-sided). c Representative histograms for Setdb1, H3K4me3, H3K9me3, and H3K27me3 expression in naïve CD4+ T cells, natural Tregs, and induced Tregs. d Mean fluorescence intensity of Setdb1, H3K4me3, H3K9me3, and H3K27me3 in naïve CD4+ T cells, natural Tregs, and induced Tregs (n = 6 per group). Unpaired Student’s t-test (two-sided). e Setdb1 CUT&Tag, H3K9me3 CUT&Tag, and RNA-seq tracks at Treg-related gene locus. Differential peaks in WT B6 mice with heart transplantation, compared to Setdb1f/fCd4-Cre mice, are displayed along with their respective P-values. Tracks indicating enhancers associated with Treg cells, as identified in EnhancerAtlas 2.0, are included as a reference track. See also Supplementary Fig. 16. f Bar plot showing the Setdb1 CUT&Tag enrichment results based on the msigdbr-C7 database from the corresponding genes determined by upregulated differential peaks in WT B6 with heart transplantation when compared to Setdb1f/fCd4-Cre mice. Hypergeometric distribution test, correction method: Benjamini–Hochberg (one-sided). g Differential peaks across each Treg cell enhancer (two-sided). h mRNA expression of Treg-related genes in splenic CD4+ T cells from WT B6 and Setdb1f/fCd4-Cre mice with heart transplantation (day 7) (n = 6 per group). Unpaired Student’s t-test (two-sided). Data are representative of at three (a, c) independent experiments. Data are shown as means ± SEM of at least three (b, d, h) independent experiments.
