Fig. 2: Cryo-EM structure of human HSL.
From: Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets
a The color-coded diagram of human HSL. The catalytic ___domain (CD) of HSL consists of three subdomains: CD1, CD2, and CD3. b The overall structure of HSL. Residues between N198 and R214, between S324 and Q337, between T491 and A638 (the majority of the regulatory ___domain), and residues before R4 and after P752 were not built in the structure. c The dimer interface between the N-terminal ___domain of one protomer and the CD2 subdomain of the other protomer. d The dimer interface between the CD1 subdomain from two HSL protomers. e Alignment of the cryo-EM structure of the catalytic ___domain of HSL with the crystal structure of the carboxylesterase EST2 (PDB: 1QZ3). The root mean square deviation (RMSD) over 1179 backbone atoms is 1.843 Å. EST2 is colored gray, with the 1-hexadecanesulfonyl group that mimics the substrate of EST2 colored yellow. The catalytic triads of HSL and EST2 are shown as sticks.
