Fig. 4: Identification of the LD-binding regions in HSL using HDX-MS.

a The workflow of the HDX-MS experiment. The ALDs were prepared using a mixture of DOPC (75%) and DOPE (25%). Created in BioRender. Hu, Q. (2025) https://BioRender.com/t74d619. b Deuterium incorporation ratios of human HSL peptides after labeling the HSL by D₂O for 5 s in the absence or presence of ALDs. The x and y axes of each point represent the central residue of a single peptide and the deuterium incorporation ratio of that peptide. The gray box indicates residues that showed higher deuterium incorporation ratios than other regions in both groups. c Changes in the deuterium incorporation ratios of human HSL upon association with ALDs were mapped on a structure of human HSL predicted by AlphaFold2 (ref. ⁴⁷). Each peptide was colored based on the change in its deuterium incorporation ratio. The changes were calculated by subtracting the incorporation ratio of each peptide in the absence of ALDs from the incorporation ratio of the corresponding peptide in the presence of ALDs. Source data are provided as a Source Data_Fig. 4 file.