Fig. 5: Residues 489–538 is the LD-binding motif in the HSL regulatory ___domain.
From: Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets
a The linear diagrams of full-length HSL and truncations of the HSL regulatory ___domain with an MBP fused to their N-termini. b, c Evaluation of the ALD-binding abilities of the MBP fusion proteins using the flotation assay. d, e Evaluation of the ALD-binding abilities of full-length HSL and HSL with residues 489-538 deleted using the flotation assay. f Subcellular localization of human HSL and its variants in 3T3-L1 preadipocytes. HSL (FL), HSL (Δ489-538), and the LD-binding motif (residues 489-538) of HSL, each with an EGFP fused to its C-terminus, were transiently expressed in 3T3-L1 preadipocytes. Then the cells were treated and imaged as described in Fig. 3e. g Quantification of the EGFP signal of human HSL and its variants on lipid droplets in 3T3-L1 preadipocytes (a total of 15 cells were analyzed from three independent measurements). The data in b, d, and f each are the results of a representative experiment out of three independent experiments. The data in c, e, and g represent the mean ± SD of three independent measurements. The data in c, e, and g were analyzed using the unpaired t-test in Prism to calculate the two-tailed P-values. Source data are provided as a Source Data_Fig. 5 file.
