Fig. 5: NpSxph rescues PtNaV1.4 block by diverse STX congeners.
From: Structural basis for saxitoxin congener binding and neutralization by anuran saxiphilins
A, B Exemplar TEVC recordings of PtNaV1.4 before (Ctrl, black) and after (red) application of 100 nM STX, and after application of Sxph in the presence of 100 nM STX at the indicated [Sxph]:[STX] molar ratios for (A) NpSxph (teal) and (B) RcSxph (orange). C [NpSxph]:[STX] (teal) and [RcSxph]:[STX] (orange) dose-response curves in the presence of 100 nM STX. D Exemplar TEVC recordings of PtNaV1.4 expressed in X. laevis oocytes in the presence of the indicated STX congener (dcSTX, GTX5, and GTX2/3) concentrations. Inset shows the voltage pulse protocol. E Toxin response curves for STX (black circles, from Abderemane-Ali et al., 2021), GTX2/3 (purple triangles), dcSTX (maroon squares), and GTX5 (magenta inverted triangles). F, G Exemplar PtNaV1.4 responses to application of (F) 200 nM GTX2/3 (red) (30 min post application), G 800 nM dcSTX (red) (one minute post application), and 3:1 [NpSxph]:[toxin] application (teal). For C and E, data represents mean with S.E.M. from 6–8 oocytes. Source data are provided as a Source Data file.
