Fig. 5: Intracellular oxidative stress eliminating, macrophage polarization effect, and biocompatibility assessment of F/R gel.
From: A dynamically phase-adaptive regulating hydrogel promotes ultrafast anti-fibrotic wound healing
a Experimental illustration of cell experiments (created with BioRender.com). b Flow cytometry of DCFH-DA fluorescence on 3T3 and HUVEC cells. c Statistical data of intracellular DCFH-DA fluorescent intensity (n = 3 independent samples). d Fluorescence images of DCFH-DA probe (green), HPF probe (green), and live/dead cell staining assay (Calcein, green and PI, red) on 3T3 cells. e The corresponding statistical analysis (n = 3 independent samples). f Flow cytometry of CD86 marker on macrophages and g the corresponding statistical data (n = 3 independent samples). h Microscopic images of red blood cells and i hemolysis rates upon various treatments (n = 3 independent samples). j Experimental illustration of subcutaneous implantation test (created with BioRender.com). k H&E staining images of skin tissues after F/R gel implantation for 28 days. l Blood routine and biochemical indexes (n = 3 biologically independent samples). Three independent experiments were performed and representative results are shown in b, d, f, h, i, k. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA with Tukey’s multiple comparison test. P value: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
