Fig. 1: Foxm1 depletion inhibits long-term tumor growth in the presence of intact immunity. | Nature Communications

Fig. 1: Foxm1 depletion inhibits long-term tumor growth in the presence of intact immunity.

From: Epigenetic silencing of DNA sensing pathway by FOXM1 blocks stress ligand-dependent antitumor immunity and immune memory

Fig. 1

a, b Representative Western blot of human FOXM1 levels in control and FOXM1 knockout MDA-MB-231 (a) or E0771 (b) cells using two different sgRNAs (sgRNA 1 and sgRNA 3). The blots are representative of three independent experiments. c Control and FOXM1 knockout MDA-MB-231 cells were plated at low density; then, cell growth/confluency measurements were performed using Incucyte assays. Results are shown as % cell confluency. d Control and Foxm1 knockout E0771 cells were plated at low density, and cell confluency measured using Incucyte assays. Results are shown as % cell confluence. Figures (c) and (d) are derived from three biological replicates, with each replicate containing two technical replicates. e Tumor volumes in NOD-scid-gamma (NSG) immunodeficient mice orthotopically implanted with scrambled control (Ctrl) or Foxm1 knockout (sgRNA 1 and sgRNA 3) E0771 cells (n = 7 mice/group). f Left: Tumor volumes in C57BL6/J immunocompetent mice orthotopically implanted with scrambled control (Ctrl) or Foxm1 knockout (sgRNA 1 and sgRNA 3) E0771 cells (n = 8 mice/group). Right: Tumor weights in scrambled control (Ctrl) and knockout (sg1 and Sg3) groups. g Left: Schema (Created in BioRender. Nirzhor, S. (2025) https://BioRender.com/s8dko5w) for rechallenge experiments to test immune memory in C57BL6/J mice implanted with scrambled control (n = 5) and Foxm1 knockout E0771 cells (n = 5). Right: Mice in the Foxm1 knockout sg3 group (n = 4) that did not form tumors were rechallenged with low passage scrambled control (Ctrl) E0771 cells at the original tumor inoculation site at day 65. Tumor volume for all experiments was calculated using V = [l x (w)2]/2. Control groups in all graphs are represented by dark blue circles, while FOXM1 knockout sg1 and sg3 groups are represented by red square or triangle, respectively. Results are displayed as mean +/− SEM. p-values for panels (c–f) (left side) were calculated using non-linear regression with Gompertz growth for panel (c, d) or Malthusian growth for panel (e, f). p-values for the panel f (right side) were calculated using Welch’s two-tailed t test. Source data are provided as a Source Data file.

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