Fig. 5: ABA-regulated global translational efficiency dependent on GRP7&8.
From: Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation
a, b Volcano plots showing changes of mRNA level (a) and translational efficiency (b) in grp7grp8CR-A compared to Col-0 seedlings under mock (left) or ABA treatment for 4 h (right). P.adjust value refers to the Benjamini-Hochberg procedure adjusted P values of two sided Wald test. c, d Venn diagram showing overlap of DEG and DTE in grp7grp8CR-A under mock condition (c), and the overlap of DTE between mock and ABA treatment in Col-0, and DTE between Col-0 and grp7grp8CR-A under mock condition (d). One-sided Hypergeometric tests were used to calculate the P values for the enrichment of genes. e The heatmap displays the log2 fold change of significant differences in translation efficiency (log2FC_TE) for two comparisons: ABA Col-0 vs Mock Col-0 and Mock grp7grp8CR-A vs Mock Col-0. The numbers represent the count of genes within each respective cluster. f Volcano plots displaying significant changes of mRNA level (left) and translation efficiency (right) in grp7grp8CR-A seedlings between mock and ABA treatment. The P.adjust value refers to the Benjamini-Hochberg procedure adjusted P-values of two-sided Wald test. g, h Venn diagram illustrating the overlap of genes whose mRNA level suppressed (g) and induced (h) by ABA treatment in Col-0 and grp7grp8CR-A. i, j Venn diagram illustrating the number of DTE between Col-0 and grp7grp8CR-A whose translational efficiency induced (i) and suppressed (j) by ABA treatment. k GO enrichment of ABA-affected and GRP7/8-dependent DTE. P.adjust value refers to the Benjamini-Hochberg procedure adjusted P-values of one-sided Hypergeometric test. l Relative mRNA levels of CA2, FBA2, CAB2 and PP2A (negative control) across different fractions of polysome profiling in Col-0 and grp7grp8CR-A/G seedlings upon mock and ABA treatment for 4 h (left). Data are the mean ± S.D. of three technical replicates from one representative polysome profiling experiment. The proportion of polysome-bound RNA (fractions 7–11, light green shade) relative to the total RNAwere displayed in bar-graph (right). Data are mean ± S.D. (n = 3 biological replicates). P value was calculated by two-sided Student’s t test. Source data are provided as a Source Data file.
