Fig. 1: Map-based cloning of HAN2.
From: Suppressing an auxin efflux transporter enhances rice adaptation to temperate habitats
a Chilling tolerance phenotype of 93-11 and NIL-HAN2NPB plants. Seedlings were incubated at 10 °C for 5 days and then transferred to normal temperature for a 7 day recovery. Upper and lower panels show 93-11 and NIL-HAN2NPB before and after chilling treatment, respectively. b Survival rates of 93-11 and NIL-HAN2NPB after recovery. Data represent mean ± SD (n = 4 biological replicates, 16 seedlings per replicate). c Local genomic structure of key recombinants and performance of their progeny after chilling treatment. CT: chilling tolerance; T: tolerant; S: sensitive; grey bars represent heterozygous regions. d HAN2 expression levels in 93-11 and NIL-HAN2NPB. Data represent mean ± SD (n = 3 biological replicates, 3 seedlings per replicate). e Chilling tolerance phenotype of WT and han2. Seedlings were incubated at 4 °C for 5 days and recovered at normal temperature for 7 days. Upper and lower panels show WT and han2 plants before and after treatment. f Survival rates of WT and han2 plants after chilling treatment. Data represent mean ± SD (n = 3 biological replicates, 24 seedlings per replicate). g Chilling tolerance phenotype of WT and HAN2-overexpression lines. Seedlings were incubated at 10 °C for 5 days and recovered for 7 days. h Survival rates of WT and HAN2-overexpression lines after treatment. Data represent mean ± SD (n = 4 biological replicates, 16 seedlings per replicate). Statistical significance in (b–h) was evaluated using a two-tailed Student’s t-test (P-values indicated). Source data are provided as a Source Data file.
