Fig. 5: CLCF1 transgenic mice exhibit enhanced muscle function and bone formation.
From: Exercise-induced CLCF1 attenuates age-related muscle and bone decline in mice
A Schematic representation of CLCF1-CRLF1 transgenic mice generation (created in BioRender.com). Male TG mice (10 weeks) were used. B, C Relative mRNA expression levels of Clcf1 (WT, n = 9; TG, n = 8; p = 0.0038) (B) and Crlf1 (WT, n = 9; TG, n = 7; p = 0.0051) (C) in TA muscle. D Plasma levels of CLCF1 protein from control and TG mice (WT, n = 4; TG, n = 5; p = 0.0240). E Weight of dissected SOL, TA, EDL, and GA muscles (WT, n = 9; TG, n = 7; biological replicates, p = 0.0096). F Grip strength test (WT, n = 9; TG, n = 7; p = 6.0e-6). G Grid hanging test (WT, n = 8; TG, n = 9; p = 0.0252). H Treadmill running performance test in mice: running curves, distance run, and time to exhaustion (WT, n = 8; TG, n = 7; p = 0.0008, 0.004, 0.003). I The number of fibers in the TA muscle (n = 4; biological replicates). J Immunostaining for myofiber boundaries using laminin in TA muscles of WT and TG mice (WT, n = 3; TG, n = 3; p = 0.0201, 0.001, 6.2e-7, 0.014, 0.005, 7.6e-7). Scale bar, 50 μm. K Glucose tolerance test in WT and TG mice (n = 9; p = 0.0494, 0.0001, 0.0318). L Representative µCT images of the trabecular and cortical compartments of distal femoral metaphyseal regions of control and TG mice. Scale bar, 1 mm. M Measurement of trabecular bone volume/total volume (BV/TV), trabecular thickness (TbTh), trabecular separation (TbSp), trabecular number (TbN), cortical bone volume/total volume (Corti BV/TV), and cortical thickness (Corti Th) (WT, n = 9; TG, n = 8; p = 0.0071, 0.0349, 0.0461, 0.0134). N TRAP, hematoxylin/eosin (H&E), and Runx2 staining of a histological section of proximal tibiae. Scale bar, 100 μm. O Osteoclast number per bone surface and osteoblast number per bone surface were assessed (WT, n = 9; TG, n = 8; p = 0.0001, 0.0067). Data are presented as means ± s.e.m.; two-tailed unpaired Student’s t-test (B–O); Log-rank (Mantel–Cox) test (H); or two-way ANOVA with post hoc Bonferroni’s multiple comparison test (K). *p < 0.05, **p < 0.005 and ***p < 0.001. Source data are provided as a Source Data file.
