Fig. 7: Exercise’s positive effects on muscle and bone are mitigated by eCNTFR.
From: Exercise-induced CLCF1 attenuates age-related muscle and bone decline in mice
A Experimental schemes for eCNTFR supplementation protocols during exercise (n = 8; created in BioRender.com). We used 12-week-old male mice. B Weight of dissected TA, GA, SOL, and EDL muscles (n = 8; p = 0.0674, 0.0754). C Grip strength test (n = 8; p = 0.0009, 0.0205). D Grid hanging test (n = 8; p = 0.0078, 0.0166). E–G Treadmill running performance test in mice: E running curves (p = 0.0025, 0.0285), F distance run (n = 7; p = 0.0014, 0.0172), and G time to exhaustion (n = 7; p = 0.0014, 0.0172). H, I mRNA expression of genes involved in fatty acid oxidation (n = 5; p = 1.21e-2, 4.89e-2, 2.3e-3, 5.0e-3, 3.0e-4, 4.0e-4, 1.0e-3, 1.2e-2, 5.0e-5, 1.5e-2, 1.4e-3, 8.0e-3, 8.0e-4) (H) and glycolysis (I) in TA muscles (n = 5, p = 8.2e-3, 8.8e-3, 1.71e-2, 4.0e-4, 6.5e-3, 2.12e-2). J Representative µCT images of the trabecular and cortical compartments of distal femoral metaphyseal regions of vehicle or eCNTFR-supplemented mice during exercise. Scale bar, 1 mm. K Measurement of trabecular bone volume/total volume (BV/TV, p = 3.0e-9, 5.0e-5), trabecular thickness (TbTh), trabecular separation (TbSp, p = 1.0e-7, 0.007), trabecular number (TbN, p = 1.0e-8, 0.0001), cortical bone volume/total volume (Corti BV/TV, p = 0.001, 0.001), and cortical thickness (Corti Th, n = 5). L TRAP, hematoxylin/eosin (H&E), and Runx2 staining of a histological section of proximal tibiae. Scale bar, 100 μm. M Osteoclast number per bone surface and osteoblast number per bone surface were assessed (n = 6; p = 0.0326, 0.0281, 0.0021, 0.0101). Data are presented as means ± s.e.m.; one-way ANOVA with post hoc Tukey’s multiple comparison test (B–M); or Log-rank (Mantel–Cox) test (E). *p < 0.05, **p < 0.005 and ***p < 0.001. Source data are provided as a Source Data file.
