Fig. 3: Employing CP6 as a scaffold to graft substrate moieties. | Nature Communications

Fig. 3: Employing CP6 as a scaffold to graft substrate moieties.

From: An mRNA-display derived cyclic peptide scaffold reveals the substrate binding interactions of an N-terminal cysteine oxidase

Fig. 3

a The crystal structure of cobalt-incorporated ADO (pink) in complex with CP6 (cyan), displayed as cylinders, highlighting the position and distance of CP6-L8 relative to the metal centre of ADO. b The chemical structure of CP6, highlighting the position of CP6-L8, the site of substrate moiety installation. c Biophysical analysis of CP6 substituents bearing a substrate moiety. (Left) Representative chemical structures of the substrate moieties grafted onto CP6 (represented as a circle) through substitutions in CP6-L8. ‘O’ corresponds to ornithine and ‘d’ corresponds to 2,3-diaminopropionic acid. (Right) SCK SPR sensorgrams of CP6 substituents bearing a substrate moiety with ADO. The sensorgrams are shown in red and the fits to the data are shown in black. The concentrations of CPs used in the titration and equilibrium dissociation constants (KD) are shown (KD given as the geometric mean of a minimum of three independent SPR measurements (n = 3)). Source data are provided as Source Data file.

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