Fig. 7: TREX-2.1 regulates DDX39B through the conserved trigger loop.
From: Structural mechanism of DDX39B regulation by human TREX-2 and a related complex in mRNP remodeling
a Structural comparison of TREX-2.1 and TREX-2M at the trigger loop interface with DDX39B. b Zoom-in view of the rectangular region in (a) with emphasis on the interaction between the trigger loop and the RecA1 ___domain of DDX39B. The models of TREX-2.1/DDX39B and TREX-2M/DDX39B were aligned on the RecA1 ___domain of DDX39B. c Trigger loop mutant of TREX-2.1 showed reduced stimulation of the ATPase activity of DDX39B. Each column represents the mean of 3 independent replicates. Error bars represent standard deviation. d Trigger loop mutant of TREX-2.1 is deficient in releasing DDX39B from RNA. This experiment has been repeated three times independently with similar results. Source Data are provided as a Source Data file.
