Fig. 3: Connectivity of the AV.
From: Distinct subnetworks of the mouse anterior thalamic nuclei
a The AV is subdivided into 4 domains, AV.medial tip, AV.medial, AV.dorsal, and AV.lateral, based on connectivity. The matrix presents Louvain-identified AV domains and shows the injection sites that group together based on the similarity of axonal or cell labeling they produce at each AV grid box. Nineteen cases (19 sections at ARA 61) were included in the analysis (injections are listed in the rows). Repeated injections for a single ROI (e.g., SUBd) were included to show they group together. Top: Recolored AV grids visualize the domains, and regions that each ___domain connects with are listed. b1 CTB in AV backlabels neurons in POST, PRE, PAR deep layers. MMl labeled cells verify the AV injection ___location. b2 Anterograde tracers in POST and PRE (labeling in LM and LD verify injection ___location) show POST/PRE/PAR (layer III)→AV.dorsal. Note the absence of labels in AD, suggesting no POST, PRE, PAR inputs to AD. c1 AV PHAL injection labels all layers of POST/PRE/PAR (AV→POST/PRE/PAR). c2 Retrograde injections in POST, PRE, PAR in a single animal reveal this as AV.dorsal→POST/PRE/PAR. d RSPd and RSPagl tracer injections show their connections with AV.dorsal (RSPv/RSPagl↔AV.dorsal). e RSPv injections show their connections with AV.lateral (RSPv↔AV.lateral). f Multiple tracer injections illustrate AV ___domain distinctions. g Retrograde tracer injection in AV.dorsal/AV.lateral validates POST/PRE/PAR→AV.dorsal and the RSPagl→AV.dorsal connections. A retrograde tracer primarily in AV.medial/medial tip labels SUBv (not POST/PRE/PAR) and RSPv (not RSPagl), corroborating the distinct SUBv/RSPv→AV.medial tip connections. h AV.medial tip ___domain receives inputs from SUBv (MM label confirms SUBv injection site) and i RSPv deep layers. j The SUBd provides input to AV.lateral. SUBd→MM projection verifies SUB injection ___location. k Cre-dependent AAV injections in SUBd (red) and SUBv (green; AAVretro-Cre in AV) show their projections to distinct AV subregions (SUBd→AV.lateral; SUBv→AV.medial tip). SUBd and SUBv projections to MM validate their injection locations54. l1 PHAL AV injection labels caudal parts of SUBv (AV→caudal SUBv), while l2 a caudal SUBv CTB injection validates the connection and demonstrates it to be with AV.lateral (AV.lateral→caudal SUBv). m Wiring diagram summarizing AV ___domain connections. n ATN-SUB wiring diagram. See Supplementary Table 2 for abbreviations.
