Fig. 3: Calcium activity is correlated with dendritic spine turnover.

a Average intensity projection of a dendritic segment and spines, color coded by calcium activity. Scale bar = 5 µm. b Smoothed calcium trace of two dendritic spines from (a). Calcium peaks (*) identified using 3 standard deviation threshold. c Calcium activity for spines that were lost, added, or retained from D1 to D5 (left) and D5 to D10 (right). D1 to D5: N = 255 lost spines, 354 retained spines, and 220 added spines, D5 to D10: N = 258 lost spines, 475 retained spines, and 250 added spines. d Cumulative distribution of calcium activity in unresponsive and responsive spines. N  = 2848 unresponsive spines, 1276 responsive spines. e Same as (c) but for responsive spines (responsive for ≥ 1 condition). D1 to D5: N = 92 lost spines (D1), 131 retained spines (D1), 64 added spines (D5), 105 retained spines (D5). D5 to D10: N = 70 lost spines (D5), 175 retained spines (D5), 58 added spines (D10), 157 retained spines (D10). f Same as (c) but for spines unresponsive across all 3 conditions. D1 to D5: N = 163 lost spines (D1), 223 retained spines (D1), 156 added spines (D5), 249 retained spines (D5). D5 to D10: N = 188 lost spines (D5), 300 retained spines (D5), 192 added spines (D10), 318 retained spines (D10). g Binoc calcium activity for spines only responsive to binoc viewing (B), spines responsive to binoc and contra viewing (B + C), spines responsive to binoc and ipsi viewing (B + I), and spines responsive to all 3 (B + C + I). D1: N = 87 B spines, 135 B + C spines, 14 B + I spines, 41 B + C + I spines. D5: 66 B spines, 105 B + C spines, 10 B + I spines, 26 B + C + I spines. D10: N = 77 B spines, 15 B + I spines, 15 B + I spines, 101 B + C spines, 44 B + C + I spines (two-way ANOVA and post hoc Tukey’s HSD for response type and day). For (c), and (e–g): error bars denote mean ± SEM. For (c–f): two-tailed Wilcoxon rank sum test. n.s. = not significant. Source data are provided as a Source Data file.