Fig. 5: 13C labeling revealed a decrease in FASII activity and an increase in lipid scavenging in absence of TgFLP12. | Nature Communications

Fig. 5: 13C labeling revealed a decrease in FASII activity and an increase in lipid scavenging in absence of TgFLP12.

From: A P5-ATPase, TgFLP12, diverging from plant chloroplast lipid transporters mediates apicoplast fatty export in Toxoplasma

Fig. 5

a %13Carbon incorporated by parasite de novo FASII in TgFLP12-iKD FA in presence and absence of ATc after 72 h incubation 8 mM 13C-U-Glucose. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). Mass isotopomer distribution of b C14:0 and c C16:0 showing the incorporation of 13C by FASII 72 h into the FA chains, M+ indicates the number of 13carbon incorporated per chain. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). d %13Carbon incorporated by parasite de novo FASII in TgFLP12-iKD FA in presence and absence of ATc of ATc after 24 h incubation with 8 mM 13C-U-Glucose after complete loss of TgFLP12 = 48 h incubation with ATc prior to 13C-U-Glucose addition. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 7). Mass isotopomer distribution of e C14:0 and f C16:0 showing the incorporation of 13C by FASII 24 h into the FA chains, M+ indicates the number of 13carbon incorporated per chain. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 7). g %13Carbon incorporated by parasite elongation in TgFLP12-iKD FA in presence and absence of ATc after 24 h incubation with 8 mM 13C-U-Acetate after complete loss of TgFLP12 = 48 h incubation with ATc prior to 13C-U-Glucose addition. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). Mass isotopomer distribution of h C16:0 and i C18:0 showing the incorporation of 13C by elongation 24 h into the FA chains, M+ indicates the number of 13carbon incorporated per chain. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). jTgFLP12-iKD FA abundances with 13Carbon incorporated by the host and scavenged by the parasite in presence and absence of ATc. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4).

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