Table 1 Oligonucleotide sequences used in this study

From: A P5-ATPase, TgFLP12, diverging from plant chloroplast lipid transporters mediates apicoplast fatty export in Toxoplasma

Oligo ID:

Sequence:

TgFLP12 pLIC Forward

tacttccaatccaatttagcgaagccggtaaagtttcgatcgc

TgFLP12 pLIC Reverse

aaccatgagattatcagactctccagg

TgFLP12 HR1 promoter replacement

tggtactcgatccttctcgacagctgaatggtgtcggcctccgccatgtttgcggatccgggg

TgFLP12 HR2 promoter replacement

cggaggcggagcggagggaggatctgttgatggctgctccatcattttgatatccctaggaattcactcgt

Reverse Cas9 plasmid

aacttgacatccccatttac

TgFLP12 gRNA Forward Cas9 plasmid

gcggctttgtcatgggagtggttttagagctagaaatagcaag

TgFLP12 Forward screen #1

caggcttggtggatgaggtac

TgFLP12 Reverse screen #2

gtcgatgtaatatacattcgcagacgg

TgFLP12 Forward screen #3

cggggagtagtcaccatgt

TgFLP12 Reverse screen #4

ccgatccagctccgacgatac

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