Extended Data Fig. 2: HESO1 confers uridylation and cytidylation of pre-miRNAs.
From: Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis
a–c, HESO1 causes pre-miRNA uridylation and cytidylation. The pHESO1:HESO1 transgene complemented the reduction of pre-miRNA uridylation in heso1-1 (a). Levels of pre-miRNA uridylation (b) and cytidylation (c) in WT (Ler) and heso1-2. Each dot represents a pre-miRNA. The black and gray lines represent the median and the quartiles, respectively. Values are Class I pre-miRNAs from one biological repeat (n = 24). Percentage of uridylation or cytidylation was calculated as [number of reads with U or C tails/number of total reads]x100. Differences between WT (Col-0) and heso1 were evaluated by Wilcox. test, two-sided, **P < 0.01. d, Site-specific uridylation analysis of Class I pre-miRNAs in WT (Col-0) and heso1-1. The diagram of positions is shown on the top. Each dot represents a pre-miRNA. The black and gray lines represent the median and the quartiles, respectively. Values are Class I pre-miRNAs from one biological repeat (n = 24). Percentage of uridylation was calculated as [number of reads with U tails at a particular position/total number of reads ending at that position]x100. Differences between WT (Col-0) and heso1-1 were evaluated by Wilcox. test, two-sided, **P < 0.01.
