Extended Data Fig. 2: RT-PCR analysis of expression of the integrated transgene pHsfA2::RbcSPTP-psbA cDNA in the transgenic lines of Arabidopsis, tobacco and rice under control or subjected to heat stress.
a, Schematic diagram of the pHsfA2::RbcSPTP-psbA cDNA construct with 2kb of HsfA2 promoter sequence and the plastid-transit peptide sequence of RbcS (RbcSPTP) fused in frame with the cDNA of the Arabidopsis plastid gene psbA at N-terminal. b-d, The expression levels of the integrated transgene pHsfA2::RbcSPTP-psbA cDNA in detached leaves of the transgenic lines of Arabidopsis (b, A1, A2 and A3), tobacco (c, T6, T13 and T58) and rice (d, R3, R13 and R23) under control or subjected to heat treatments (for Arabidopsis, 41 °C, 1 h; Tobacco, 42oC, 1 h; Rice, 44oC, 1 h) using the specific primers (RbcSPTP-F and psbA-R), derived from the plastid-transit peptide sequence RbcSPTP and coding region of the psbA cDNA respectively by RT-PCR analysis. No corresponding fragments could be amplified from the wild-type samples. Three times these experiments were repeated independently with similar results.
