Extended Data Fig. 2: Identification of Arabidopsis pdk1.1 and pdk1.2 T-DNA insertional mutants.
From: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis
a, Schematic representation of PDK1.1 and PDK1.2 genes and positions of T-DNA insertions for pdk1.1 and pdk1.2. Introns, exons, and non-coding regions are indicated by lines, black, or blank boxes respectively. Positions of primers are indicated. b, Identification of homozygous pdk1.1 and pdk1.2 mutants. Genomic DNA of pdk1.1 and pdk1.2 mutants was used as templates for PCR amplification. Homozygous lines have a single amplified DNA fragment when using LBa1/pdk1.1-RP or LBa1/pdk1.2-RP primers. n = 5 biologically independent experiments, with similar results obtained. c, qRT-PCR analysis confirmed the deficient expression of PDK1.1 and PDK1.2 genes in pdk1.1 and pdk1.2 mutants, respectively. Total RNA of 7-day-old WT, pdk1.1 and pdk1.2 seedlings was extracted, reversely transcribed, and then used for analysis. ACTIN7 was amplified and used as an internal reference to normalize the expression of PDK1.1 and PDK1.2, and the mean value in Col-0 was set as “1”. The experiments were biologically repeated for 3 times. Dots represent individual samples, and lines indicate mean ± s.d.. P values were calculated by a Welch’s two-tailed t-test.
