Extended Data Fig. 1: Characterization of AtCXE15-OE plants and atcxe15 mutants.
a, Transcript abundance of AtCXE15 in the shoot and root explants upon CIM treatments. Log2-transformed relative transcript values of AtCXE15 is calculated by comparing transcript level of AtCXE15 at each time points to 0 h. Magenta, green and white indicate upregulation, downregulation, and no change, respectively. b, Characterization of AtCXE15-OE plants carrying a p35S:AtCXE15:GFP construct. The primary rosette branch number, transcript abundance of AtCXE15, and AtCXE15-GFP and Actin protein levels in the corresponding transgenic plants are shown from upper to bottom panels, respectively. The GFP-tagged AtCXE15 and endogenous Actin levels were detected by immunoblotting with a monoclonal anti-GFP and anti-actin antibody, respectively; the dilution rate for both antibodies is 1: 2,000. c, Molecular characterization of the T-DNA insertion mutant atcxe15-1. The T-DNA insertion site and positions of the primers used RT-PCR are indicated by black triangles and red lines, respectively. The experiment was repeated at least two times independently with similar results. d, The mutations in atcxe15 alleles generated by the CRISPR/Cas9 system. The CRISPR/Cas9 targeting sites on AtCXE15 are shown in blue letters followed by red protospacer adjacent motif (PAM), and allelic mutations in the atcxe15-2, atcxe15-3, and atcxe15-4 are shown as indicated. e, Quantified rosette primary shoot branch number in the three atcxe15 plants described above. The means of branch number are shown as black lines in b and e; n = 3 biological replicates of 10 plants for each genotype. The qRT–PCR data were normalized to the Col-0 and are shown as means ± s.d. in b; n = 4 biological replicates of 16 plants for each genotype. The statistical analysis was performed by comparing Col-0 with each AtCXE15-OE line or atcxe15 genotype; F (degree of freedom) values are shown; The letters in b and e indicate statistical differences between genotypes determined by one-way ANOVA followed by Tukey’s HSD test for multiple comparisons (P < 0.05).
