Extended Data Fig. 1: Temperature and CLV3p receptors intersect to buffer auxin mediated floral primordia formation.
From: A network of CLAVATA receptors buffers auxin-dependent meristem maintenance
a-e, CLE receptors and temperature intersect to buffer floral primordia formation. a, Quantification of flower primordia termination at 16 °C (n = 9–21), 23 °C (n = 10–24) and 30 °C (n = 12–15) for Col-0, pol, crn, crn pol, clv1-101, clv1-101 crn, clv1-101 pol, clv1-101 crn pol, cik124, cik124 crn, cik124 pol, cik124 crn pol. b, Inflorescence images at 16 °C and 30 °C of clv1-101, clv1-101 crn pol, cik124, and cik124 crn pol. All cik alleles in this figure are cik1-3, cik2-3, and cik4-3 CRISPR generated alleles. Same magnification within temperatures. c, Quantification of flower primordia termination at 16 °C (n = 8–9), 23 °C (n = 8–13), and 30 °C (n = 12) for Col-0, pol, crn, crn pol, clv1-8 crn, clv1-8 pol, clv1-8 crn pol. d, Inflorescence images at 16 °C, 23 °C and 30 °C of clv1-8 crn and clv1-8 crn pol. Same magnification within temperatures. e, Maximum intensity projection of DR5::GFP (green fire blue LUT) and PI (grey) and DR5::GFP alone in the IMs pol (n = 7), clv1-101 pol (n = 6), and clv1-101 crn (n = 8). f, Maximum intensity projection of Col-0 CLV1pro::2xYPET-N7 (green) inflorescence meristem. Expression patterns of CLV1pro::2xYPET-N7 in Col-0 (n = 4) and crn pol (n = 5) showing no expression changes. g, Expression patterns of POLpro::YPET-H2AX (green, n = 6) in Col-0 and WUSpro::POL-YPET (green, n = 3) and DR5::GFP (purple) in crn pol showing L5 and Z axis (PI, red). h, Diagram of flower/fruit production on primary inflorescence in wild-type and terminating and recovering crn plants. n = X biologically independent samples and statistical groupings based on significant differences using Kruskal-Wallis and Dunn’s multiple comparison test correction (a, c) where significance is defined as p-value < 0.05. Scale bars 10 mm (b, d) and 20 µm (e, f, g).
