Extended Data Fig. 2: CIK co-receptors are required for temperature-dependent floral primordia outgrowth.

a, b, CIK receptors act redundantly in temperature sensitive floral primordia formation. a, Quantification of flower primordia termination at 16 °C (n = 7–14), 23 °C (n = 8–9) and 30 °C (n = 7–9) for Col-0, crn, cik1, cik2, cik3, cik4, cik12, cik13, cik14, cik234, cik124, cik134, cik123 and cik1234. b, Inflorescence images at 16 °C of cik1, cik12, cik13, cik14, cik124, cik134, cik123, cik1234 with arrows pointing to termination. Same magnification within single and double mutants as well as within triple and quadruple mutants. All cik alleles in this figure are cik1-1, cik2-1, cik3-1, and cik4-1 T-DNA lines. c. CIK genes are expressed in the IM. Expression patterns of YPET-H2AX (green fire blue LUT) reporter lines in the IM with XY view of L5 and axial view of same stack indicated with dotted line of CIK1pro (n = 10), CIK2pro (n = 15), CIK3pro (n = 8) and CIK4pro (n = 14) in Col-0 showing representative images (PI, magenta). n = X biologically independent samples and statistical groupings based on significant differences using Kruskal-Wallis and Dunn’s multiple comparison test correction (a) where significance is defined as p-value < 0.05. Scale bars 10 mm (b) and 20 µm (c).