Extended Data Fig. 5: LoVIL1 interacts with PRC2 complex but not LoNFYC6.

a, Validation of the interaction between LoVIL1 and PRC2 subunit LoMSI1 using yeast two-hybrid (Y2H) assay. AH109 yeast cells harbouring LoVIL1 and LoMSI1 were cultured on SD-TL (SD-Trp-Leu) and SD-TLH (SD-Trp-Leu-His) selecting media. Images were taken after 3 days of culture. At least five individual yeast colonies were tested for each construct, and one representative was shown. BD was used as the negative control; BD-53 was used as the positive control (same procedures in panels c and e). b, Split-luciferase complementation assay confirming the interaction between LoVIL1 and LoMSI1. The ORF of LoVIL1 was cloned into the nLUC vector, and LoMSI1 was cloned into the cLUC vector. cLUC is used as the negative control. Luciferase activities were determined by luminescence imaging after 3 days of N. benthamiana leaf agroinfiltration. Color bars indicate luminescence intensity from weak (blue) to strong (red or white). Three biological replicates were performed with consistent results (same procedures in d and f). c, Validation of the interaction between LoNFYA7 and LoNFYC6 using Y2H assay. AH109 yeast cells harbouring LoNFYA7 and LoNFYC6 were cultured on SD-TL and SD-TLH + 1 mM 3-AT selecting media for 3 days. d, Confirmation of the interaction between LoNFYA7 and LoNFYC6 using Split-luciferase complementation assay. e, LoVIL1 does not interact with LoNFYC6 in Y2H system. AH109 yeast cells harbouring LoNFYA7 and LoNFYC6 were cultured on SD-TL and SD-TLH + 1 mM 3-AT selecting media for 3 days. f, LoVIL1 does not interact with LoNFYC6 in the Split-luciferase complementation system.