Extended Data Fig. 2: LC-MS/MS analysis of AZG1.
From: Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1
a, AZG1 expression detected by whole-cell western blot using anti-Flag tag antibody. n = 3 independent experiments. b, Typical UHPLC-MS/MS chromatograms for the analysis of tZ. c, Typical UHPLC-MS/MS chromatograms for the analysis of tZ-d5. d, Typical UHPLC-MS/MS chromatograms for adenine standards and adenine extracted from WT and mutant AZG1 protein samples. e, Comparison of the peak area of adenine extracted from WT and mutant AZG1 protein samples. Data are mean ± s.d., n = 3 biologically independent samples. Statistical analysis was performed using two-tailed t-tests. P = 0.0000685 for D393F, P = 0.0001124 for T440Y, and P = 0.0000450 for D393W/T440W double mutant. Differences were considered statistically significant at P < 0.05.
