Extended Data Fig. 3: Structure determination of AZG1apo/pH7.4, AZG1adenine/pH5.5, and AZG1T440Y/apo/pH7.4.
From: Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1
a, Size-exclusion chromatography of AZG1apo/pH7.4 on Superose 6 (GE Healthcare) and SDS-PAGE analysis of the final sample. n = 3 independent experiments. b, Representative cryo-EM micrograph and 2D classification images of AZG1apo/pH7.4. n = 3 independent experiments. c, Flowchart of image processing for AZG1apo/pH7.4. d, The density map of AZG1apo/pH7.4 colored by local resolution. e, Angular distribution plot of particles included in the final C2-symmetric 3D reconstruction of AZG1apo/pH7.4. f, The Gold standard Fourier Shell Correlation (FSC) curve of the final 3D reconstruction, and the FSC curve for cross-validation between the map and the model of AZG1apo/pH7.4. g-l, Equivalent panels to the ones described above, for the structure determination of AZG1adenine/pH5.5. For g and h, n = 3 independent experiments. m-r, Equivalent panels to the ones described above, for the structure determination of AZG1T440Y/apo/pH7.4. For m and n, n = 3 independent experiments.
