Extended Data Fig. 2: Verification and transcriptomic characterization of ΔruvC mutants.
a, Verification of the ΔruvC knockout mutant. Determination of the genotype by PCR using three pairs of primers that amplify the full-length RuvC gene, the RuvC upstream fragment with spectinomycin (Spec) gene, and the partial fragment of fliC gene which encodes R. solanacearum flagellin, respectively. b, Detection of RuvC-HA expression level in three RuvC complementary strains (ComRuvC) by western blotting. Total proteins were analyzed by an α-HA antibody and the loading was shown by Coomassie brilliant blue (CBB) staining. c, Heatmap showing the correlation between each sample calculated by Pearson correlation coefficient. d, Scatter plots of whole-genome transcript fragments per kilobase of transcript per million mapped reads in GMI1000 versus ΔruvC knockout mutant. The x-axis indicates gene expression in GMI1000 and the y-axis indicates gene expression in ΔruvC knockout mutant.
