Fig. 2
From: Circular RNA expression is abundant and correlated to aggressiveness in early-stage bladder cancer
Profiling of circular RNAs in human bladder cancer. a Heatmap of all detected circRNAs (supported by ≥two reads, n = 38,365) and their expression level across samples. Rows represent unique circRNAs and have been sorted by number of samples with expression of each. Samples are sorted by circRNA expression level within each row. Black tick mark indicates 20,819 unique circRNAs that are only supported by two reads in one sample. The small heatmap (right) gives and overview of all samples and the larger heatmap (right) shows the expression distributions for the 400 most abundantly expressed circRNAs. b The number of backspliced reads supporting distinct circRNAs (supported by ≥ two reads in ≥ two different samples, n = 15,223). Y-axis is plotted on a logarithmic scale (log10). c The number of samples expressing distinct circRNAs. Y-axis is plotted on a logarithmic scale (log10). d Number of total circular reads per gene versus number of distinct circRNAs per gene. e Number of circular and corresponding linear reads supporting each candidate. X-axis and Y-axis are plotted on a logarithmic scale (log10). There are 303 circRNAs with no support of linear splicing, including ciRS-7. f Genomic origin of bladder cancer circRNAs and linearly spliced transcripts. Upper panel shows fraction of splice sites and lower panel shows fraction of reads and hence combined expression level of circRNAs from each region. Relative levels of circular (red) and linear (blue) splicing can be compared. 3′-UTR: 3′-untranslated region, 5′-UTR: 5′-untranslated region, CDS: coding sequence, ncRNA: non-coding RNA. g Number of exons composing exonic circRNAs (n = 14,569). h Exonic length of circRNAs (n = 14,569)
