Fig. 3: Lipidomics analysis showing differences in lipid classes and lipid species composition. | npj Parkinson's Disease

Fig. 3: Lipidomics analysis showing differences in lipid classes and lipid species composition.

From: Impaired neuron differentiation in GBA-associated Parkinson’s disease is linked to cell cycle defects in organoids

Fig. 3

a Differences in phosphatidylethanolamine (PE) and phosphatidylcholine (PC) levels but not hexosylceramides (HexCer), sphingomyelins (SM) or phosphatidylinositol (PI) in HILIC LC-MS/MS based lipidomic analysis of DIV30 organoids. Data represent a summary of four independent organoid differentiations, Mann–Whitney U test. **p < 0.01, ****p < 0.0001. Error bars represent standard error of the mean. b Pairwise comparison between GBA-PD vs control of phosphatidylinositol (PI) lipid species denoted by their sum notation. Data expressed as the Log2 of the fold change. Error bars represent standard error of the mean. c Plots for PI molecular species PI (38:5), PI (36:1), PI (40:4), each plot corresponds to the most enriched species for that sum notation: (18:1/20:4), PI (18:0/18:1), and PI (18:0/22:4), respectively. Data represent a summary of four independent organoid differentiations and is expressed as the percentage of the entire PI class. p-values were calculated using a one-way ANOVA test. FDR adjusted p-values were calculated using the Benjamini–Hochberg procedure. **p < 0.01, ****p < 0.0001. Error bars represent standard error of the mean. d Fatty acid composition of the lipid species within the sphingomyelin lipid class. Data represent a summary of four independent organoid differentiations expressed as relative quantification, where each species is normalized to the total amount of lipid of its class. p-values were calculated using a one-way ANOVA test. FDR adjusted p-values were calculated using the Benjamini–Hochberg procedure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars represent standard error of the mean.

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