Fig. 2: nbn is the mutated gene of lvv mutant. | npj Regenerative Medicine

Fig. 2: nbn is the mutated gene of lvv mutant.

From: The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway

Fig. 2

a Genetic map of the candidate region on chromosome 16. Numbers above SSLP markers indicate recombination events. b Sequencing results displaying the mutation site in nbn. c Schematic diagram of the structure of Nbn protein. FHA, BRCT, and NLS denote forkhead-associated ___domain, BRCA1 C-terminal ___domain, and nuclear localization signal, respectively. d Schematic illustration of Mtz treatment and analysis at R48h. e Single optical section images showing Myc (red), Dendra2 (green), and DAPI (blue) staining in regenerating livers at R48h under the Tg(lfabp:DenNTR) background. Note that Myc-tagged NbnWT is mainly localized in the nucleus, while Myc-tagged Nbncq137 is localized in the cytoplasm. f WISH images showing nbn expression in the liver region (arrows) from BT to R48h. Numbers indicate the proportion of larvae exhibiting the expression shown. Scale bars, 100 μm. WT wild-type, DAPI 4′, 6-diamidino-2-phenylindole, BT before Mtz treatment, R regeneration time after the withdrawal of Mtz, WISH whole-mount in situ hybridization.

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