Fig. 2: Human donor-derived in vivo myofibers are more transcriptionally mature than in vitro-differentiated myotubes.

a Heatmap displaying expression values (logCPM) for all differentially-expressed genes (DEGs) between in vitro-differentiated myotubes and human donor-derived myofibers in NSG mice (“in vivo myofibers”). Hierarchical clustering was performed to group samples and genes by similarity, resulting in four unique clusters of genes arose. High expression in orange, low expression in teal. b Tables describing all muscle-related pathways enriched in Cluster 1 and all significant pathways enriched in Cluster 2 after pathway analysis (DAVID, GOTerm Biological Processes). c Bubble-plot depicting the top pathways that emerge when all DEGs are included in pathway analysis (Reactome) for in vitro myotubes versus in vivo myofibers. d Bar graphs showing the log-fold change (logFC) of genes that are annotated to three different gene sets that emerged as top pathways. Enriched genes in orange and downregulated genes in teal. e Heatmap of expression (logCPM) for major myogenic genes in in vitro myotube and in vivo myofiber samples. Gray bars on the left side highlight the development stage associated with each gene (1 - myogenic commitment, 2 – differentiation, 3 – immature myofiber, 4 – adult myofiber). High expression is in orange and low expression in teal, where color is based on row minimums/maximums. Light blue shading represents instances where no human transcripts were measured for that sample (n = 2 and n = 3 for in vitro myotube and in vivo myofiber samples, respectively).