Fig. 3: The myogenic signature of in vivo myofibers strongly corresponds with adult human skeletal muscle, whereas in vitro myotubes more closely resemble fetal human muscle.
From: The adult environment promotes the transcriptional maturation of human iPSC-derived muscle grafts
a Table depicting the sample information pertaining to the human biospecimens selected from the ENCODE Project database for comparison with iPAX7-derived samples. b PCA plot of human skeletal muscle (termed “fetal SkM”, “child SkM”, and “adult SkM”) as well as human in vitro myotubes and in vivo myofibers (n = 10 fetal SkM, n = 2 child SkM, n = 12 adult SkM, n = 2 in vitro myotube, and n = 3 in vivo myofiber samples). c Heatmap of expression (logCPM) for major myogenic genes comparing human skeletal muscle biospecimens (“fetal”, “child” or ”adult”) against in vitro myotube and in vivo myofiber samples. Gray bars on the left side highlight the development stage associated with each gene (1 - myogenic commitment, 2 – differentiation, 3 – immature myofiber, 4 – adult myofiber). High expression is in orange and low expression in teal, where color is based on row minimums/maximums. d Heatmap of expression (logCPM) for other muscle-related genes involved in skeletal muscle structure, comparing human skeletal muscle against in vitro myotube and in vivo myofiber samples. High expression is in orange and low expression in teal, where color is based on row minimums/maximums.
