Fig. 4: In vivo validation: Early and transient fiber cross-linking modulation guides tissue repair outcomes. | npj Regenerative Medicine

Fig. 4: In vivo validation: Early and transient fiber cross-linking modulation guides tissue repair outcomes.

From: A computational model reveals an early transient decrease in fiber cross-linking that unlocks adult regeneration

Fig. 4

a Morphological image of AT after scarring repair (Vehicle), upon transient inhibition of cross-links (BAPN), under regenerative conditions (Nal-M), and upon transient increase of cross-links (Nal-M + Genipin). Images were taken 1-month post-injury. b Box-plot of light transmittance after Vehicle, BAPN, Nal-M, and Nal-M + Genipin treatments. c Representative fluorescence (300 µm thick tissue sections) images of regenerative (Nal-M and BAPN) and scarring conditions (Vehicle and Nal-M + Genipin) 1-month post-injury. The two lines represent the reconstructed area. Red, blue, and green colors correspond to adipocytes, nuclei, and collagen respectively. Scale bars represent 2,000 µm (panel a) and 100 µm (fluorescence images). d Histogram of the reconstructed area (%) based on the ratio of adipocyte area compared to the whole reconstructed area upon regenerative and scarring repair. e Boxplot of fractal dimension under regenerative, scarring, and BAPN conditions 3 days post-injury. Data are expressed as mean +/- SEM. Data analyzed by Tukey’s test. *p < 0.05; **p < 0.01; ****p < 0.0001.

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