Fig. 3

Serine/threonine Pamgene kinome array analysis of pooled control (C, n = 12) and schizophrenia (S, n = 12) run in the presence or absence of specific inhibitors (i) for AKT, JNK, MEK, and PKC. a Heat map showing the ratio of signal intensity of the sample with inhibitor/sample without inhibitor. Lighter to darker blue represents decreased phosphorylation (inhibition) on a specific array peptide, while lighter to darker red indicates increased phosphorylation (activation). Comparison of left and right sides in the column shows differential kinase activity and phosphorylation of peptide substrates between control and schizophrenia. b Differential phosphorylation by inhibitor type. Black circles represent peptide substrates with a difference in fold change of greater than 0.5, in which directionality (kinase activity increased, decreased, or not changed) was different between schizophrenia and control samples. White circles represent peptide substrates in which differences in fold change were greater than 0.5 but activity changed in the same direction in both samples. Gray circles represent peptide substrates in which the difference in fold change was less than 0.5 regardless of whether kinase activity was increased, decreased or unchanged on peptide substrates in both samples. c, d Representative enzyme kinetic curves for peptides substrates that were differentially phosphorylated by AKT (c) or JNK (d) inhibitors