Fig. 5: The immune profile induced by Ad26.RSV.preF in neonatal animals deviates significantly from the profile induced by FI-RSV. | npj Vaccines

Fig. 5: The immune profile induced by Ad26.RSV.preF in neonatal animals deviates significantly from the profile induced by FI-RSV.

From: Adenovector 26 encoded prefusion conformation stabilized RSV-F protein induces long-lasting Th1-biased immunity in neonatal mice

Fig. 5

Neonatal mice (n = 6) received a single immunization with 1010 vp Ad26.RSV.preF at Day 4 or 5 after birth, or 3 weeks later. Control groups were not immunized (indicated with -), or received a prime-boost immunization with FI-RSV. IgG1 (left panel) and IgG2a (right panel) subclass RSV F-binding antibodies were determined in serum taken 9 weeks a or 25 weeks b after prime immunization. c A subgroup of animals was sacrificed 9 weeks post-prime-immunization and splenocytes were isolated and stimulated with RSV F peptide pools. IFN-γ, IL-4, IL-5, and IL-10 secretion in the supernatant of F-stimulated splenocytes was determined by a multiplex ELISA-based analytical method. Ratios between Th1 (IFN-γ) and Th2 (IL-4, IL-5, or IL-10) were calculated for samples with at least one of the values above background levels (95th percentile of unstimulated splenocytes). Means a and b and geometric means c per group are indicated with horizontal lines. Statistical analysis was performed with Tobit regression and Dunnett or Dunnett–Hsu correction.

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