Fig. 3: Creation of nanoparticles displaying optimized immunogens.
a Antigen optimization process first reverting designed amino acids (red) back to WT identity (gray), followed by display on a 24-copy ferritin nanoparticle (blue). b Screening of optimized immunogen monomers. Nickel purification yields are reported for all constructs. Tm was measured for those constructs with sufficient yields. Immunogens 15-2 and 17-4 were identified as optimized leads for nanoparticle display. c SDS-PAGE analysis of purified immunogens fused with ferritin. d Size-exclusion chromatogram of purified particles after freeze thaw, indicating formation of a stable particle. e Negative-stain EM micrographs of purified nanoparticles displaying optimized immunogens. Scale bars are 20 nm. The 2D class averages are shown below each micrograph.
