Fig. 2: Cryo-EM analysis of ConM, ConM-EDC, ConS, and ConS-EDC. | npj Vaccines

Fig. 2: Cryo-EM analysis of ConM, ConM-EDC, ConS, and ConS-EDC.

From: Profound structural conservation of chemically cross-linked HIV-1 envelope glycoprotein experimental vaccine antigens

Fig. 2

a Side views of unmodified ConS (red) and ConM (blue) in complex with PGT122 Fab (gray) at 3.1 Å and 3.4 Å respectively. Each cryo-EM map is shown at high (colored map) and low (light outline) thresholds to highlight the PGT122 constant ___domain and N-linked glycans. b ConM and ConS structures were superimposed, and local Cα RMSD was rendered onto the ConM structure with Chimera, according to color and to the thickness of the main chain cartoon representation. Color scales represent local Cα RMSD in Angstrom (Å). c Same as in b but comparing ConS-EDC and ConM-EDC structures rendered onto the ConS-EDC structure. d Same as in b, comparing ConS and ConS-EDC structures, rendered onto the ConS structure. e Same as in b, comparing ConM and ConM-EDC rendered onto the ConM structure.

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