Fig. 8: Splenocytes isolated from immunized BALB/c mice in each group on day 35, and T-cell responses were detected by flow cytometry after stimulation of the spleen with virus A/PR/8/1934 (PR8) or A/Aichi/2/1968 (X-31).

Shown are detailed results of CD8⁺ IFN-γ⁺ T cells (A), CD8⁺ IL-4⁺ T cells (B), CD8⁺ TNF-α⁺ T cells (C), CD8⁺ IL-2⁺ T cells (D), CD4⁺ IFN-γ⁺ T cells (E), CD4⁺ IL-4⁺ T cells (F), CD4⁺ TNF-α⁺ T cells (G), and CD4⁺ IL-2⁺ T cells (H) induced by virus PR8 and detailed results of CD8⁺ IFN-γ⁺ T cells (I), CD8⁺ IL-4⁺ T cells (J), CD8⁺ TNF-α⁺ T cells (K), CD8⁺ IL-2⁺ T cells (L), CD4⁺ IFN-γ⁺ T cells (M), CD4⁺ IL-4⁺ T cells (N), CD4⁺ TNF-α⁺ T cells (O), and CD4⁺ IL-2⁺ T cells (P) induced by virus X-31. Data are presented as the mean with standard error (SEM) (n = 4; one-way ANOVA with Tukey multiple comparison), *P < 0.05, **P < 0.01.