Fig. 5: Cross-neutralization of VOC-matched pseudoviruses by sera from CoroVax vaccinated mice.
Sera from BALB/c mice were tested against pseudoviruses bearing B.1 (D614G), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), B.1.1.529.1 (Omicron BA.1), B.1.1.529.2 (Omicron BA.2), and B.1.1.529.5 (Omicron BA.5) spikes, and the calculated ID50s are depicted in the graph. The dashed line indicates the limit of detection. The differences in neutralization of different variant viruses are indicated by horizontal lines, and the fold differences in neutralization GMTs are shown. Mice were immunized with a single injection of (a) CorovaxG.3, (b) CorovaxG.3-P, (c) CorovaxG.3-D, (d) CorovaxG.3-D.FR, (e) CorovaxG.3-O.FR. For prime/boost experiments mice were immunized with a dose of CorovaxG.3 followed by a boost 28 d later of either (f) CoroVaxG.3, (g) CoroVaxG.3-D.FR or (h) CoroVaxG.3-O.FR. i Antigenic map of SARS-CoV-2 VOCs based on sera of mice vaccinated with a single dose of CoroVax candidates. SARS-CoV-2 variants are displayed as circles and sera are represented by squares. Each square corresponds to serum of one animal. Each grid square represents 1 antigenic unit and equals a 2-fold change in neutralization titer. (j) Antigenic map of SARS-CoV-2 VOCs based on sera of mice vaccinated with a prime/boost regimen of CoroVax candidates. The circles more closely together evidence higher cross-neutralization. ID50s used to build these maps were extracted from Supplementary Table 1. k Cross-neutralization titer was calculated as the GMT against all VOCs except the matched strain. The box plots shows the median, 25th and 75th percentiles, and the whiskers show the range.
