Fig. 7: cHA-ΔNS1-LAIV vaccination activates tissue-resident memory (TRM) IAV-NP⁺CD8⁺ T-cells on day 3 post-infection.

Four weeks post-final-boost, mice were challenged with a QIV matched IVR-180 using a lethal dose of 100 LD₅₀. a A representative image of the gating strategy used to probe lung resident NP-tetramer+ CD8⁺ T-cell responses of animals on day 3 post-infection. The following markers were utilized for the gating strategy; CD3e—T cell marker, CD8a—CD8 T cell marker, CD69—Activation marker, CD44—Memory marker, CD103—Tissue resident marker (absent in CD4+) b Absolute count of TRM IAV-NP⁺CD8⁺ T-cells 3 days post infection. Each dot is one animal and data are shown as mean ± SEM Statistical significance was compared to QIV standard of care group (Group 7) using one-way ANOVA using Dunnett’s correction. **** P- <0.0001.