Fig. 4: Bivalent mRNA vaccine M1.2 protected mice from Omicron subvariants challenge.
A Quantity of Omicron BA.1 virus outgrown from lungs of mice. Mice in Fig. 1B were challenged intranasally with 105 TCID50 Omicron BA.1 virus. Columns indicate median TCID50/100 mg lung tissue, and error bars indicate minimal and maximal values of TCID50/100 mg lung tissue. Numbers above the boxes are median values of TCID50 per 100 mg lung tissue (n = 10). Each dot represents an individual mouse. B Quantity of viral genomic RNA copies in tissues from mice in A. Columns indicate median viral genomic RNA copy numbers per 100 mg tissue (for lung and nasal turbinate) or per swab sample (for oropharyngeal swabs), and error bars indicate minimal and maximal values of the viral genomic RNA copy numbers (n = 10). Each dot represents an individual mouse. C Quantity of Omicron BA.5 or XBB.1.5 virus outgrown from lung tissues or Oral swab & Nasal wash samples from mice. Mice in Fig. 3A were challenged intranasally with 105 TCID50 Omicron BA.5 virus (n = 5) or Omicron XBB.1.5 virus (n = 4–5). Columns indicate median PFU per lung or per sample, and error bars indicate minimal and maximal values of the PFU. Each dot represents an individual mouse. D Correlation between the quantity of viruses outgrown from mouse samples and nAb ID50 titers. Each dot represents one mouse. One-way ANOVA (Kruskal-Wallis test) followed by Dunn’s multiple comparison were used for statistical analysis, except for oropharyngeal swab in B and Oral swab & Nasal wash in C where two-way ANOVA followed by Tukey’s multiple comparison were used. Spearman correlation was used in the correlation analysis in D. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
