Extended Data Fig. 6: Maintenance of wrinkle treatment via serial injection of COL1A1-EVs and COL1A1-EV MN.
From: Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy
a, After 8 weeks of UV irradiated photoaging, wrinkles were tracked for mice treated every 30 days with 1) saline, 2) COL1A1-EVs, and 3) COL1A1-EV MN on days 0, 4, 7, 14, 21, 28, 49, 70, and 91 (COL1A1-EVs, COL1A1-EV MN, Saline). n = 4, Scale bar, 5 mm. b, Total wrinkle number (n = 4 for all groups; *P = 0.019 COL1A1-EV MN vs Saline at day 4; #P = 0.034 COL1A1-EVs vs Saline, *P = 0.031 COL1A1-EV MN vs Saline at day 7; #P = 0.030 COL1A1-EVs vs Saline, **P = 0.008 COL1A1-EV MN vs Saline at day 14; **P = 0.008 COL1A1-EV MN vs Saline at day 21; *P = 0.025 COL1A1-EV MN vs Saline at day 28; **P = 0.006 COL1A1-EV MN vs Saline at day 35; #P = 0.031 COL1A1-EVs vs Saline, *P = 0.030 COL1A1-EV MN vs Saline at day 42; #P = 0.044 COL1A1-EVs vs Saline, *P = 0.016 COL1A1-EV MN vs Saline at day 49; *P = 0.016 COL1A1-EV MN vs Saline at day 56; #P = 0.017 COL1A1-EVs vs Saline, *P = 0.020 COL1A1-EV MN vs Saline at day 63; *P = 0.018 COL1A1-EV MN vs Saline at day 70; #P = 0.011 COL1A1-EVs vs Saline, **P = 0.006 COL1A1-EV MN vs Saline at day 77; #P = 0.028 COL1A1-EVs vs Saline, *P = 0.037 COL1A1-EV MN vs Saline at day 84; #P = 0.043 COL1A1-EVs vs Saline, *P = 0.043 COL1A1-EV MN vs Saline at day 91) and c, wrinkle area on the dorsal skin of the mice during 90 day study window (n = 4 for all groups; #P = 0.012 COL1A1-EVs vs Saline, **P = 0.005 COL1A1-EV MN vs Saline at day7; #P = 0.010 COL1A1-EVs vs Saline, *P = 0.048 COL1A1-EV MN vs Saline at day 14; #P = 0.023 COL1A1-EVs vs Saline, *P = 0.021 COL1A1-EV MN vs Saline at day 21; *P = 0.022 COL1A1-EV MN vs Saline at day 28; *P = 0.046 COL1A1-EV MN vs Saline at day 35; #P = 0.019 COL1A1-EVs vs Saline, **P = 0.009 COL1A1-EV MN vs Saline at day 42; #P = 0.042 COL1A1-EVs vs Saline, *P = 0.030 COL1A1-EV MN vs Saline at day 49; *P = 0.030 COL1A1-EV MN vs Saline at day63; *P = 0.029 COL1A1-EV MN vs Saline at day 70; #P = 0.048 COL1A1-EVs vs Saline, *P = 0.022 COL1A1-EV MN vs Saline at day 77; #P = 0.040 COL1A1-EVs vs Saline, *P = 0.027 COL1A1-EV MN vs Saline at day 84; *P = 0.045 COL1A1-EV MN vs Saline at day 91). All data are from three independent experiments and are presented as means ± SEM. Two-way ANOVA was used for the comparisons in (b, c).
